polyclonal anti his tag antibody  (Cell Signaling Technology Inc)

Journal: PLOS One

Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells

doi: 10.1371/journal.pone.0339375

Figure Lengend Snippet: (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.

Article Snippet: For antibody affinity testing, LILRB4 (hFc-tagged) and CD16A (6His-tagged) recombinant proteins(TargetMol, USA) were coated onto 96-well plates at 50 ng/well and incubated overnight at 4°C.

Techniques: SDS Page, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Flow Cytometry, Binding Assay, Cell Culture, Expressing, Bioprocessing, Co-Culture Assay

Journal: BMC Infectious Diseases

Article Title: Development and evaluation of novel Brucella diagnostic antigen protein ST

doi: 10.1186/s12879-025-12393-1

Figure Lengend Snippet: Validation of ST protein expression and purification. A . SDS-PAGE electrophoresis results. M: Marker; Lane 1: Before IPTG induction; Lane 2: Supernatant of 0.5 M IPTG-induced at 37 °C; Lane 3: Supernatant of 1 M IPTG-induced at 37 °C; Lane 4: Supernatant of 0.5 M IPTG-induced at 25 °C; Lane 5: Supernatant of 1 M IPTG-induced at 25 °C; Lane 6: Supernatant of 0.5 M IPTG-induced at 16 °C; Lane 7: Supernatant of 1 M IPTG-induced at 16 °C; Lane 8: Deposition of 0.5 M IPTG-induced at 37 °C; Lane 9: Deposition of 1 M IPTG-induced at 37 °C; Lane 10: Deposition of 0.5 M IPTG-induced at 25 °C; Lane 11: Deposition of 1 M IPTG-induced at 25 °C; Lane 12: Deposition of 0.5 M IPTG-induced at 16 °C; Lane 13: Deposition of 1 M IPTG-induced at 16 °C. B . Western blot (WB) results of purified HIS tag protein. M: Marker; Lane 1: Purified recombinant protein ST protein

Article Snippet: Finally, the purified ST protein was electrophoresed on a 10% SDS-PAGE gel (Bio-Rad, Hercules, USA; Cat. 161–0183), transferred to a PVDF membrane (Millipore, Burlington, USA; Cat. IPVH00010), washed three times with TBST buffer containing 0.05% Tween 20 (Amersham Biosciences, Little Chalfont, UK; Cat. GE2000) for 10 min each time, and subjected to Western blotting with rabbit anti-His tag polyclonal antibody (Proteintech, Rosemont, USA; Cat. 13844-1-AP) and HRP-labeled goat anti-rabbit IgG antibody (Santa Cruz Biotechnology, Dallas, USA; Cat. sc-2004).

Techniques: Biomarker Discovery, Expressing, Purification, SDS Page, Electrophoresis, Marker, Western Blot, Recombinant